Singleron offers a complete single-cell sequencing service with focus on clinical researches, based on the SCOPE™ single cell kits, software and meaningful annotation of data. The intergreted results allow clinicians a new comprehensive view on molecular correlations and progression of certain diseases. Until today more than 8000 samples (surgical samples, biopsies, PBMC and others) from over 350 different tissue types have been successfully sequenced and analyzed.
Single Cell Sequencing Service, clinical meaningful analysis
|Sample Type||Appearance||Weight||Status||Temperature||Storage duration|
|Tissue||no leak, label intact, within temperature range||≥100mg||fresh tissue||2-8℃||<72h|
|Fresh Blood（EDTA）||no leak, label intact, within temperature range||≥5mg||no hemolysis||2-8℃||<72h|
|Biopsy||no leak, label intact, within temperature range||≥3 pieces||fresh biopsy||2-8℃||<72h|
When we started with our single-cell RNAseq study of osteosarcoma, we encountered a challenge immediately after acquiring the tumor samples: it was nearly impossible to be dissociated to highly viable single cell suspension that is required for single cell sequencing, even with multiple attempts using in-house and commercial kits. Following suggestions from a colleague, we used Singleron sCelLiVE Tissue Dissociation Solution which could beautifully dissociate osteosarcoma samples with a straightforward protocol. This made it possible for us to move forward successfully with our project which resulted in an osteosarcoma single-cell atlas showing cellular heterogeneity and potential therapeutic targets and a publication in Nature Communications.
Dr. Haiyan Hu
Chief Physician, Oncology Department
Shanghai Jiao Tong University, The Sixth People’s Hospital Shanghai, China
Phone：+49 221 16824777
E-mail：firstname.lastname@example.org (German office)
Address：Singleron Biotechnologies GmbH, Gottfried-Hagen-Straße 60, 51105 Köln, Germany