Our proprietary GEXSCOPE™ tissue processing solution makes it easy to preserve, transport, and dissociate tissue samples for single cell sequencing. The GEXSCOPE™ (Single Cell Omics Preparation Entity）Tissue Preservation Solution can preserve cell viability in fresh tissue at 4℃ for up for 72 hours, making it easy to store or transport samples to central laboratory for further processing. The innovative GEXSCOPE™ (Single Cell Omics Preparation Entity）Tissue Dissociation Master Mix comprises a mixtures of different enzymes in an optimized buffer system which can effectively break down tissues to generate single cell suspension with >90% viable cells within an hour, without requirement for special equipment. The Dissociation Master Mix has already been successfully tested in over twenty tumor and normal tissue types.
Ⅱ：Single Cell Separation and Sequencing Library Construction:
Cell suspension can be manually loaded onto GEXSCOPE™ chip(Single Cell Omics Preparation Entity）, a portable, closed-format microwell chip to settle single cells into individual wells. Magnetic beads with error-correcting barcode design can subsequently be loaded into the wells to capture and label mRNA from each single cell with a unique cell barcode. Reverse transcription, cDNA amplification, and library construction are then performed with optimized GEXSCOPE™ (Single Cell Omics Preparation Entity）enzymes and buffers.
Ⅲ：Bioinformatics Analysis and Data Mining:
Singleron is building a unique clinical single cell database that integrates cellular and molecular information with clinical insights. The database contains a large number of datasets from single cell RNAseq samples processed with GEXSCOPE™ (Single Cell Omics Preparation Entity）system, as well as datasets from public databases and relevant publications. Combining with multidimensional information on genes, pathways, and drug responses, we are establishing machine-learning models to accurately annotate cell populations and obtain clinically relevant insights automatically.
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